Extreme high moderate low section 1 chemical product and company identification product name water, depc treated. Information in this sds is from available published sources and. Depc will react with primary amines and cannot be used directly to treat tris buffers. Nuclease free water our nuclease free water is available as either depc treated or non depc treated water. For this study, we tested transcription reactions for depc inhibition effects. All rna solutions should be prepared using at least 0. Water, depctreated, molecular biology grade cas 7732185 calbiochem cas 7732185 sterilefiltered water treated with diethyl pyrocarbonate depc. Diethyl pyrocarbonate depc is a chemical use to inactivate rnase enzymes and is sensitive to moisture and ph. Autoclave the solution to inactivate the remaining depc. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. Ambion depctreated water is certified nucleasefree and supplied in ten bottles containing 50 ml each. Immediately flush eyes with large amounts of water for at least 20 minutes adequate flushing is achieved by separating eyelids with clean fingers. If one does not wish to autoclave, depc treated water. Also our dnasernase free distilled water is not treated with depc.
This safety data sheet was created pursuant to the requirements of. Depc treated water 1 ml t7 promoter forward primer 0. Water, depctreated, molecular biology grade cas 773218. Water, depc treated is a depc diethylpyrocarbonate treated and sterile filtered water. Other companies are charging thousands of dollars to set up accounts and give you access to. Material safety data sheet 910 clopper road gaithersburg, maryland 20878 usa. View product specific information, msds, references and buying faq. Material safety data sheet water, depc treated sc204391 hazard alert code key.
Safety data sheet for nucleasefree water e7431 neb. Ricca r9145000 molecular biology grade water, depc treated. Ultrapure depctreated water thermo fisher scientific. Safety summary see msds for complete information before using product. Data sheet for information regarding hazards and safe handling practices. Is it okay to use water while diluting cdna or primers. Product codeam9922 product name depc treated water ml. Water, rnase free, endotoxin testeddepc treated safety data sheet according to federal register vol. Product code detector pcr dna biotinylation kit 600101. Thermo scientific deionized, diethylpyrocarbonate depc treated and 0.
Diethyl pyrocarbonate depc is an efficient, nonspecific inhibitor of rnases. If one does not wish to autoclave, depc treated water may be boiled at 100 c for. Duplicate maxiscript transcription reactions were assembled using 32putp, and the same concentration of depc treated water. Shake vigorously, and allow the solution to sit overnight in a fume hood.
Depc reacts with amine, hydroxy and thiol groups of proteins thereby inactivating rnases and other enzymes. Kd medical inc rgj0050 depc treated and therefore rnasefree water is used in handling of rna in the laboratory to reduce the risk of rna. Show this safety data sheet to the doctor in attendance. Diethyl pyrocarbonate depc, also called diethyl dicarbonate iupac name, is used in the laboratory to inactivate rnase enzymes in water and on laboratory utensils. Perform the depc treatment as described above, and add the amine to the autoclaved solution once the solution has cooled. Previously we used to use rnase free water depc water for all genetic experiments that is rna extraction, cdna synthesis, semipcr or rt. These are available as convenient pdf files online at. Although autoclaving does inactivate depc by causing hydrolysis of depc. Contaminated packagingdo not reuse empty containers. Ultrapure depctreated water is suitable for use with rna. Depc is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.
Highlights free of endo, exodeoxyribonucleases, ribonucleases, and phosphatases. Call a poison control center or doctor for treatment. It is typically used to treat water and solutions before working with easily degraded rna. Water, rnasefree, depc treated while this material is not considered hazardous by the osha hazard communication standard 29 cfr 1910. Sterile depc ultrapure water material safety data sheet 16. Eye contact hold eye open and rinse slowly and gently with water for 15 20 minutes. This molecular biology grade water from ricca chemical comes in a 1 l petg clear container and is nuclease free, protease free, sterile, and depc treated. What is the protocol of using depc in rna extraction. It decomposes at 155c, to ethanol and carbon dioxide in aqueous solution. Material safety data sheet sterile depc ultrapure water. Depc treated water safety overview national diagnostics. Other information for research use only, not for human or veterinary clinical use. When preparing tris buffers, treat water with depc first, and then dissolve tris to make the appropriate buffer. Template dna was dried to completion in a vacuum centrifuge and resuspended in 0.
Water, depc treated is used for the reconstitution of lyophilized products such as oligomers, proteins, and sirna. Waste treatment methods the generation of waste should be avoided or minimized wherever possible. Depc has been reported to be incompatible with polycarbonate or polystyrene containers. Remove contact lenses, if present, after the first 5 minutes, then continue rinsing eye. Note depctreated water is not recommended for pcr or in vivo experiments. Gene link depc treated water and nuclease free water depc free has been treated and autoclaved water that are certified to be free of nucleases and nucleic acids. Download msds pdf search msds currently available for download bioworld offers depc for your research at low price. Depc inactivates rnase enzymes in water by covalent modifications of the histidine residues. Kd medical inc depc ultra pure water, 20l depc ultra.